ZFIN Image: Feng et al., 2010, Fig. S1

Image

Figure Caption

Fig. S1 V12RAS induces pro-inflammatory gene up-regulation in zebrafish larvae. (A) RT-PCR showing up-regulation of pro-inflammatory genes in V12RAS⁺ larvae at 4dpf compared with their V12RAS^- siblings. (B) qPCR showing increased expression of il1² and cxcl1 in 5-dpf hsp:V12RASeGFP larvae compared with WT after both have been heat shocked for 6 h. (C) Fluorescent in situ hybridization of arginase1 (cyan) combined with L-plastin antibody staining for leukocytes (magenta) and anti-RAS antibody staining for V12RAS⁺ cells (green) in 7-dpf V12RAS⁺ larvae. (D) Anti-TNFα antibody staining (cyan) combined with anti-L-plastin antibody staining for leukocytes (magenta) in 7-dpf larvae with V12RASeGFP⁺ clones (green)-arrowheads indicate TNFα signal inside some of the L-plastin⁺ cells. *, p<0.05. Scale bars = 20 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.