Fig. 4 Distinguishing four leukocyte subtypes in zebrafish larvae and their response to a wound versus that to the presence of transformed cells.
(A) Features of four leukocyte subtypes, as illustrated by typical examples from Tg(fli:eGFP, LysC:DsRed)larvae (Video S6). (B) (i) Tracks of each leukocyte subtype from a movie (Video S6A) of the wound inflammatory response in a Tg(fli:eGFP, LysC:DsRed) larva, illustrating how each responds to a wound. Faint white line indicates the wound edge. (ii) Similar tracking data from a movie (Video S7B) of a Tg(kita:GalTA4, UAS:V12RASeGFP, fli:eGFP, LysC:DsRed) larva showing that all subtypes of leukocyte are responding to V12RAS+ cells. Scale bars: (A), 16 μm; (B), 50 μm.