Fig. 3 Spry4 controls the onset of krox20 expression. (A-C) Zebrafish embryos injected with either control morpholino (Ctrlmo) (A), Spry4mo (B) or Spry2mo (C) were collected at 100% epiboly and subjected to in situ hybridisation with krox20 and ntl probes (purple). Arrows in B indicate krox20 expression in a few r5 cells. The insets show tailbud views of the embryos, allowing determination of the developmental stage by evaluation of the closure of the tailbud, as revealed by ntl expression. (D-G) Distribution of embryos showing either no krox20 expression, limited expression in r3 or expression in both r3 and r5 at 90, 95 or 100% epiboly or at 10.25 hours post-fertilisation (hpf). ns, not significant; *, P<0.05; **, P<0.0001; χ2-test.
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