ZFIN Image: Seebald et al., 2011, Fig. S3

Image

Figure Caption

Fig. S3 Analysis of eve1 transcripts from uninjected and eve1-MO alone injected AB/AB and TL/TL wild-type embryos at 6 hpf by RT-PCR. A Lane 1) eve1 cDNA template used as positive control. A Lane 2) No template as negative control. A Lane 3) RT-template from uninjected AB/AB wild-type embryos. A Lane 4) l.0-kb DNA markers. A Lane 5) RT-template from AB/AB embryos injected with 6 ng eve1-MO. B Lane 1) l.0-kb DNA markers. B Lane 2) RT-template from uninjected TL/TL wild-type embryos. B Lane 3) RT-template from TL/TL embryos injected with 6 ng eve1-MO. Black arrow indicates the expected normal PCR product of 674-bp. Arrowhead indicates the longer PCR product of 895-bp that contains the sequence of intron 1.

Acknowledgments

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Reprinted from Developmental Biology, 349(1), Seebald, J.L., and Szeto, D.P., Zebrafish eve1 regulates the lateral and ventral fates of mesodermal progenitor cells at the onset of gastrulation, 78-89, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.