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Fig. 3 ColXIX knock down phenocopies the stumpy mutation. (A) RT-PCR shows inclusion of a 109 bp fragment in splice-blocking colXIX MO injected (MO) compared to wild-type (wt) embryos. (B-D) CaP axon phenotypes as visualized using znp-1 antibody in (B) uninjected wild-type embryos, (C) wild-type embryos injected with 9 ng colXIX MO and (D) stumpyb393-/- mutant embryos. White dashed line indicates the horizontal myoseptum. Scale bar: 70 μm.
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