ZFIN Image: Yin et al., 2010, Fig. 6

Image

Figure Caption

Fig. 6 Loss of Hand2 Function Reduces MMP Activity along the LPM/Gut Boundary

(A) Diagram showing the microinjection of the DQ-collagen IV substrate into the embryo. Lateral view, anterior to the left. The blue dot marks the position where gut looping occurs.

(B) Tg(hand2:EGFP) embryos were injected with DQ-collagen IV at 26 hpf, fixed at 27 hpf, and stained for GFP (blue) and phalloidin (red). The fluorescein signal caused by cleavage of the DQ-collagen substrate is shown in green.

(C) In embryos preinjected with GM6001, almost no fluorescein signal could be detected.

(D and E) Wild-type (D) and han^c99-/- (E) embryos were coinjected with DQ-collagen and Alex549-conjugated collagen at 27 hpf and fixed at 28 hpf. The spots where the fluorescence intensity was measured are marked by pink and orange bars.

(F) MMP activity represented as normalized fluorescence intensity (mean ± SEM). Six wild-type and six han^c99 mutants were examined at each time point. Asterisks indicate statistical significance: ^*p < 0.05.

(B–E) Transverse sections, dorsal to the top. L, left; NC, notochord; NT, neural tube; R, right; So, somite. The scale bars represent 40 μm.

Acknowledgments

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Reprinted from Developmental Cell, 18(6), Yin, C., Kikuchi, K., Hochgreb, T., Poss, K.D., and Stainier, D.Y., Hand2 Regulates Extracellular Matrix Remodeling Essential for Gut-Looping Morphogenesis in Zebrafish, 973-984, Copyright (2010) with permission from Elsevier. Full text @ Dev. Cell