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Fig. 4
Temporal and spatial analyses of cx30.3 mRNA expression. A: Expression of cx30.3 was detected at the 1 day post fertilization (dpf) stage, and thereafter maintained throughout embryonic development. The zebrafish ef1α gene was used as positive control to demonstrate the integrity and genomic-DNA-contamination-free status of the cDNA samples. B:cx30.3 mRNA is predominantly expressed in the adult skin, fin, inner ear, ovary, liver, heart, retina, while a relatively low level of cx30.3 expression was also detected in the gill, swim bladder, skeletal muscle, spleen, and gut. The relative level of mRNA expression was calculated by comparing ΔCt between mean Cts of zebrafish cx30.3 and ef1α.