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Fig. 1
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Fig. 1
EMT markers are up-regulated during zebrafish heart regeneration in vivo. (A) RT-PCR analysis of EMT markers. Expression of snail1a, snail1b, snail2, twist1a, and twist1b was examined in sham and 7-, 10-, and 14-dpa hearts by semiquantitative RT-PCR. RT-PCR of β-actin is included as a loading control. (B) snail2 ISH in sham and 7- and 10-dpa hearts. (Scale bar = 100 μm.) (C) twist1b ISH in sham and 10- and 14-dpa hearts. The dashed line marks the approximate position of the amputation plane. (Scale bar = 100 μm.)
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Full text @ Proc. Natl. Acad. Sci. USA