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Fig. S2 Solubility of the mutant c-myb DNA binding domain in vitro. (A) Purification of c-myb proteins from E. coli lysates after Ni-Sepharose affinity chromatography. Lane 1, wild-type protein; lane 2, mutant protein; lane 3, 60 kDa size standard (BSA); lane 4, molecular weight standards; sizes in kDa are indicated at the right. Silver stain. (B) Solubility of c-myb proteins was tested by Western blotting. Note that the CD buffer causes the protein to aggregate.
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