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Fig. S8 Muscle patterning is grossly normal in srn mutants. Slow muscle fibers were examined with F59 antibody and glia cells in the spinal cord were examined with GFAP antibody. While there is an obvious reduction of GFAP+ glia cells in the spinal cord in srn mutants, the patterning of slow muscle fibers is similar in srn and wild type embryos at 48 hpf. Previous work showed that fast muscle fiber number and patterning are unaltered in srn compared to wild type embryos at 48 hpf (Panzer et al., 2005; 3 embryos, 1 carrier pair were examined). Scale bar = 200 μm.
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