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Fig. 7 A. qRT-PCR assessment of fold change in hes5, her4 and heyl expression in WT, srn and mib mutant embryos at 48 hpf, normalized to β-actin1. hes5, her4 and heyl expression is dramatically reduced in srn, similar to those in mib, but to a lesser extent. (3–5 experiments, 20 embryos each, one-way ANOVA, Bonferroni′s Multiple Comparison Test, ** p<0.001, * p<0.5). B. hes5, her4 and heyl in situ hybridization at 48 hpf confirm reduced expression in the brain and spinal cord in srn mutants compared to WT (>30 embryos for each). Scale bar = 100 μm.
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