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Fig. S5 Expression pattern of PTCH1 in zebrafish embryos. (A) Distribution of zebrafish Ptch1 transcript during early developmental stages shared some similarities with that of GRK2. Wild-type embryos were collected at the indicated time points and WISH was performed by using the Ptch1 riboprobe as described by Hogan et al. [Hogan BM, et al. (2005) Duplicate zebrafish pth genes are expressed along the lateral line and in the central nervous system during embryogenesis. Endocrinology 146:547–551]. (B) In situ hybridization result of zebrafish Ptch1 in 24-hpf control and GRK2 morphants.
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