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Fig. S2 Early expression patterns in the diencephalon. Her6 is expressed in the entire anterior neural plate at tailbud stage (a). The expression domain overlaps with the marker of the posterior forebrain wnt8b shown by a dorsal view. A fluorescent analysis shows that both markers overlap in the area of the posterior diencephalon, shown by quenching of the red fluorescence (a′ and a′′). At the 5-somite stage, her6 is detectable in the telencephalon and in the posterior diencephalon, shown by co-localization with wnt8b in a lateral view (b). At the 10-somite stage, her6 is expressed in the entire thalamic complex including the thalamus anlage marked by irx1b in a lateral view (c). At the 24 hpf stage, expression of the proneural gene neurog1 (d) is first observed in the ventro-posteriro doemain of the irx1b+ positive Th. Notably, we find neurog1 in the basal plate outside to the alar located MDO (asterisk). ascl1b marks the Pth, the rTh and the PTec at 33 hpf (e). Fb, forebrain; Mb, midbrain; PTh, prethalamus; rTh, rostral thalamus; tel, telencephalon; Th, thalamus.