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Fig. 5 Normal development and locomotion of double plxnb2a and plxnb2b morphants. A: Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of the efficiency of the double knockdown using B2aMO and B2bMO morpholinos interfering with the splicing. Each morpholino was used at a concentration of 5 ng per embryo. E6* = 32 end of exon 6. B: Light microscopy images of lateral views of the double morphant as well as the controls at 5 days postfertilization (dpf). Ventral views of Alcian blue-stained 5 dpf double morphant and controls visualizing the cartilage structure. C-E: Results of the swimming performance assay from one representative replicate (zebrafish larvae per group n = 16-27). Graphs of the total distance moved (C), velocity (D), and angular velocity (E) are represented as mean and standard error of mean.