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Fig. S4 foxj1a and rfx2 are up-regulated in zebrafish cystic kidney mutants. (A and B) In WT embryos at 56 hpf, both foxj1a and rfx2 were expressed in a “salt-and- pepper” distribution characteristic of multiciliated pronephric duct cells. Expression of foxj1a and rfx2 was up-regulated in all cells of the pronephric duct in the dilated pronephric ducts (arrowheads) of 56-hpf embryos homozygous for fleer (C and D), oval (E and F), tg292a (G and H), and double bubble (J) mutations, all of which developed pronephric cysts by 56 hfp, as well as in pkd2 morphants (I). No increase in foxj1a expression over control was observed in pronephros of mutants before cystic stretch (36 hpf,) shown here for the schmalhans cilia cystic mutant (K and L).