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Fig. S1 Cells other than adipocytes contain lipid. A: Cryosections of adult zebrafish (12-18 months) were sectioned (30 μm) and stained in oil red O. Small lipid droplets are present in hepatocytes, skeletal muscle, and chondrocytes (black arrow heads), but not in mucous cells (black arrows), which have a similar morphology to adipocytes. Scale bar = 50 μm. B: Whole-mount oil red O staining of a 5 dpf larvae show lipid accumulation in several tissues, including the yolk, liver, jaw, and brain, but no large unilocular lipid droplets are observed in areas that coincide with regions of the fish that contain cells with adipocyte morphology. Scale bar = 1 mm.