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Fig. 1 Id2a knockdown results in microphthalmia and defects in retinal lamination. (A,B) Id2a-MM-injected (control) (A) and Id2a-MO-injected (B) zebrafish embryos at 48 hpf display a reduction in eye and brain size. (C-H) Transverse histological sections of Id2a-MM and Id2a-MO retinas at 48 (C,F), 72 (D,G) and 120 (E,H) hpf. Id2a-MO eyes are smaller than Id2a-MM eyes, and the inner nuclear layer (INL) and outer nuclear layer (ONL) are not morphologically identifiable in Id2a-MO retinas at 72 hpf (G). (I) Measurements of absolute eye size along the anteroposterior (A/P) and dorsoventral (D/V) axes at 48 hpf. n=6 embryos; ***, P<0.001. Error bars indicate ± s.e.m. Dorsal is up in all images. GCL, ganglion cell layer; L, lens. Scale bars: 150 μm in A,B; 80 μm in C-H.