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Fig. 5 Lefty1,2 expression after suppression of sox17 and of chd in DFC. Embryos were injected into the yolk at one-cell (A–E) or midblastula (F,G) stages, with CO MO (A and E-G), sox17 MO (B-D and E,F), or chd MO (G) as indicated. Whole mount in situ hybridization for lefty1 and/or lefty2 at 22hpf (A-D: arrows; dorsal view with anterior to the left) showed aberrant expression when either sox17 MO or chd MO were injected, as summarized in E-G. Expression of lefty1 in the brain and lefty2 in the heart field was scored, as indicated (E, F) in sox17 MO-injected embryos, but only lefty2 was scored after chd MO injection (G). L, left; B, bilateral; R, right; A, absent. Number of embryos is shown at the top.