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Fig. 2 Injection of sox17 MO leads to abnormal pancreas placement, which is rescued by sox17 RNA co-injection. Embryos injected at the one-cell stage with 10 ng CO MO, or 10 ng sox17 MO alone or combined with sox17 RNA as indicated, were in situ hybridized with insulin at 3dpf. A-C: Sox17 MO injected embryos showed normal expression on the right side (A), and abnormal opposite expression on the left (B); dorsal views, anterior to left. Some embryos showed fragmented ins-positive domains (C left, arrows), with an example of an intact domain in the right panel (lateral views, anterior to right). D: Localization of ins expression after injection of MOs with or without RNA, as indicated. R, right; M, middle; L, left; f in Rf, Mf, or Lf refers to fragmented ins-positive domains. Number of embryos is shown at the top.