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Fig. 3 Overexpression of Id2a leads to macrophthalmia but does not affect retinal lamination. (A,B) mgfp mRNA-injected (control) (A) and id2a mRNA-injected (B) zebrafish embryos at 48 hpf. Approximately 58% of embryos injected with id2a mRNA exhibit enlarged eyes compared with embryos injected with mgfp mRNA. (C) Quantification of eye size in mgfp mRNA-injected and id2a mRNA-injected embryos at 48 hpf. n=5 embryos; **, P=0.015. (D-G) Transverse histological sections of mgfp mRNA and id2a mRNA-injected embryos at 48 (D,E) and 72 (F,G) hpf. The id2a mRNA retina at 48 hpf (E) is noticeably larger than the mgfp mRNA-injected control (D). Retinal lamination is normal at both time points. Error bars indicate ± s.e.m. Scale bars: 150 μm in A,B; 80 μm in D-G.