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Fig. S8 Monitoring mouse Myc-Dmbx1 and zebrafish Myc-dmbx1a levels in vivo. Representative images of an (A) un-injected, (B) mouse myc-Dmbx1 mRNA injected (400 pg), and (C) zebrafish myc-dmbx1a mRNA injected (150 pg) tailbud stage embryo (lateral view) processed for whole-mount immunolabeling using an anti-Myc antibody (n = 10 per group). Panels D-F are representative high magnification images of embryos in each of the three treatment groups; black arrows indicate nuclei that are DAB+ in the injected groups, but lack DAB staining in the un-injected group.
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Full text @ BMC Dev. Biol.