Fig. 8

Fig. 8 atoh1a and atoh1b cross-activation and establishment of a focal FGF signaling center. (A,B) atoh1MOs eliminate deltaD (A), but not deltaA (B), expression in the pLLp of wild-type zebrafish embryos. (C) SU5402 treatment eliminates deltaA expression after 2 hours. (D) atoh1a expression is retained in the trailing proneuromasts following 15 minutes of SU5402 exposure (middle), but is lost by 30 minutes (bottom). (E) atoh1b knockdown reduces atoh1a expression in the trailing neuromast (top). atoh1b knockdown accompanied by SU5402 exposure for 15 minutes reduces atoh1a expression throughout the entire pLLp (bottom). (F) atoh1b expression is restricted to the trailing neuromast. (G) atoh1b expression expands in mib1^ta52b pLLps (top) but is lost when atoh1a MO is injected (bottom). (H) knockdown of atoh1a expands atoh1a expression at the leading end, but expanded expression is not maintained at the trailing end (bottom).