Fig. 2

Fig. 2 poky encodes chuk/ikk1/ikkα. (A) Schematic of domains of Ikk1. Amino acid sequence around the T-loop activation domain in wild type; poky mutant and human sequence is aligned below. The site of the missense mutation in poky^p20ad is indicated by the asterisk (*). T-loop activating serines are indicated in red. Missense mutation in poky^p20ad substituting the conserved phenylalanine for serine is indicated in green. Nonconserved amino acids in the human sequence are indicated in blue. (B) Percent rescue to 24 hpf by ikk1 mRNA. (C,D,E,H,I,J) Whole mount in situ hybridization for cyt1 (C,H), krt8 (D,I) and krt4 (E,J) at 50% epiboly. (F,G,K,L,M–V) whole mount in situ hybridization for krt4 at 50% epiboly following microinjection of the indicated mRNA. (W–Y) Cell autonomy of Ikk1. (W) Anti myc staining of myc-Ikk1 scatter labeled embryo. (X) krt4 expression of the same embryo. (Y) Merged image of W and X. Rescued EVL cells (arrowhead) and isolated myc positive DEL cells (arrow) can be observed.