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Fig. 3 The pro-BMP activity of CV2-T5P/I21R requires the presence of Chordin.
(A) Graphical illustration of proportions of dorsalized and ventralized phenotypes generated upon injection of cv2-T5P/I21R or bmp2b mRNA (in blue letters), cv2, chd or nog1 MOs (in red letters), or mixtures of the mRNA/MOs in different combinations. Numbers of analyzed embryos are indicated above the columns; columns are numbered. (B–J) Representative zebrafish embryos after mRNA and/or MO injection as in (A). Right panels show overall morphology of live embryos in lateral views at 32 hpf, left panels show embryos at 80% epiboly (mid gastrula stage), after whole mount otx2/eve1 double in situ hybridizations (domains indicated in B), lateral views, dorsal to the right. The expression of the dorsal marker otx2 is reduced in ventralized and expanded in dorsalized embryos, whereas the ventral marker eve1 shows contrary shifts.