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Fig. S1 Expression of tfap2d is absent from developing melanophores. Lateral views of wild-type zebrafish embryos, fixed at the stage indicated and processed to reveal tfap2d expression by RNA in situ hybridization. (A) At 24 hpf, an embryo shows tfap2d expression within specific regions of the midbrain, (B) which persists until 36 hpf. Importantly, tfap2d expression is not detected within the trunk of these embryos. Embryos were treated with low levels of PTU to decrease melanin production to allow better visualization of potential expression within melanophores. Scale bar: 50 μM.
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