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Fig. 5 Polyubiquitylated proteins are localized in lens fiber cell nuclei. (A-F) Labeling of wild-type (A-C) and vov mutant (D-F) zebrafish lens of the indicated stages with anti-polyubiquitylated protein antibody (FK2, green) and TOPRO3 (red). Arrowheads indicate signals in nuclei of differentiating lens fiber cells at 72 hpf (C). Scanning conditions of images in D,F were the same as those for B,C. (E) The same image as D but with reduced sensitivity of fluorescence detection. (A′-F′,A"-F") The same images as A-F but shown through a green (FK2) or red (TOPRO3) channel. Scale bars: 50 µm.
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