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Fig. S1 Rnd3 interacts with the N-terminal of Syx. 293T cells were co-transfected with SBP-Flag Rnd3 along with the indicated Flag-tagged Syx constructs. Cells were lysed 24 h post-transfection, and cleared lysates were subjected to SBP pulldown. The purified protein complex was analyzed by immunoblotting with anti-Flag antibody to visualize the associated proteins.
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