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Fig. S3 Apical specification at the Kupffer′s vesicle is largely unaffected in tri mutant and bb8-morphant. Visualisation of apical membrane and cilia in the Kupffer′s vesicle using anti-ZO-1 and anti-α-acetylated tublin antibodies in the control (A), tri-/- (B), bbs8-morphant (C) and tri-/- injected with bbs8-MO (D) embryos. ZO-1 was observed on the cell membrane lining the KV (A-D), suggesting that apical specification is mostly intact in all conditions. Also note that the near-complete oval outline of the KV in wild-type (A) is partially disrupted in B-D (Yen et al., 2006).
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Reprinted from Developmental Biology, 345(2), May-Simera, H.L., Kai, M., Hernandez, V., Osborn, D.P., Tada, M., and Beales, P.L., Bbs8, together with the planar cell polarity protein Vangl2, is required to establish left-right asymmetry in zebrafish, 215-225, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.