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Fig. 3 Vacuoles fuse with luminal membranes during initial lumen expansion. (A) An ISV from a living Tg(flk1:moesin1-egfp)/Tg(flk1:nlsmCherry) zebrafish embryo with the box indicating the area shown in B. (B) Time-lapse confocal images showing formation of primary lumen in an ISV with the integration of vacuoles (arrows) from 30 hpf. (C,D) A dorsal ISV from a living Tg(flk1:moesin1-egfp)/Tg(flk1:nlsmCherry) embryo injected intravenously with labeled dextran (D) showing the intracellular vacuoles without labeled dextran (C, arrows). The time is in minutes:seconds. n, nucleus.
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