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Fig. S1 Knock-down of nav1.6a leads to migration of DRGs in the anterior and posterior trunk. (A,B) Three separate siRNAs targeting scn8aa increase the number of ectopic DRG neurons in 4 dpf Tg(ngn1:GFP) compared with uninjected embryos (*P<0.05 versus uninjected wild type; **P<0.01 versus uninjected wild type; Kruskal-Wallis nonparametric ANOVA). siRNAs were synthesized by Dharmacon (Lafayette, CO, USA) and had the following sequences: siRNA1, GGAGAATGGTGGTACTAAT; siRNA2, GGACCTGGCTATCACCATT; siRNA3, GGCCATGTGTCTCATCGTA. All three siRNAs reduce the touch response, similar to the 1.6 morpholino (not shown). (C) Although the majority of ectopic DRG neurons are found in segments 2-4 in wild type and siRNA-injected embryos, nav1.6a knock-down increases the number of ectopic DRG neurons in segments 2-4, 5-7 and 8-13 (***P<0.01 versus wild-type neurons in the same segments; two-tailed t-test).