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Fig. 4 Developmental RT-PCR profile of wnt9a mRNAs. Amplification of β-actin (587 bp) was run in parallel to standardize input cDNA. RT-PCR amplification of a region of the wnt9a ORF (696 bp) reveals increasing expression levels after zygotic gene expression begins at 3 hpf peaking around 2 dpf then leveling off. Expression is maintained in mature zebrafish, indicated by the juvenile (1.5 months). Q-PCR analysis reveals that the highest level of expression is approximately 200x greater than the expression at 6 hpf which is normalized to 1. Negative control is no RT.
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Reprinted from Gene expression patterns : GEP, 10(6), Cox, A.A., Jezewski, P.A., Fang, P.K., and Payne-Ferreira, T.L., Zebrafish Wnt9a,9b paralog comparisons suggest ancestral roles for Wnt9 in neural, oral-pharyngeal ectoderm and mesendoderm, 251-258, Copyright (2010) with permission from Elsevier. Full text @ Gene Expr. Patterns