ZFIN Image: Rhodes et al., 2010, Fig. 5

Image

Figure Caption

Fig. 5 Reduced myca expression in rad21^nz171 mutants and smc3 morphants. A, B, expression pattern of myca in whole-mount wild type and rad21^nz171 embryos at 24 h.p.f. and 36 h.p.f. respectively (anterior to the left). myca expression (purple) in the brain and eye of wild type is absent in rad21nz171 embryos. C, D, Expression of myca is also greatly reduced in smc3 morphants at 24 h.p.f. and 36 h.p.f. respectively (anterior to the left). Embryos were injected with antisense morpholino oligonucleotides targeting the start codon (smc3ATG-MO) or the 3′ donor site of exon 1 (smc3Spl1-MO) of the smc3 gene to create two smc3 morphants. E, F, The expression of myca in smc3 morphants (smc3ATG-MO and smc3Spl-MO) is significantly reduced compared to wild type embryos as measured by quantitative PCR at 24 h.p.f. and 36 h.p.f. respectively.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 344(2), Rhodes, J.M., Bentley, F.K., Print, C.G., Dorsett, D., Misulovin, Z., Dickinson, E.J., Crosier, K.E., Crosier, P.S., and Horsfield, J.A., Positive regulation of c-Myc by cohesin is direct, and evolutionarily conserved, 637-649, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.