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Fig. 5 Nlz1 represses expression of the luciferase transgene and promotes histone deacetylation at the transgenic promoter in TG(UAS-SV40:Luciferase) embryos.A, B. TG(UAS-SV40:Luciferase) embryos were injected with mRNA encoding GAL4DBD or Nlz1-GAL4DBD as indicated under the graph. Embryos were raised for 12 hours and assayed for luciferase activity (A) or for histone H4 acetylation at the transgenic promoter (B) as described in the Methods section. Data are expressed as fold luciferase activity (A), or fold H4 acetylation (B) relative to un-injected control. A T-test indicates p < 0.0005 for the difference in acetylation level between GAL4DBD- and Nlz1GAL4DBD-injected embryos.