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Fig. 8 gata2 and gata3 expression in the lateral floor plate requires Nkx2 genes but not tal2. (A,B) Zebrafish embryos injected with a mix of mismatch morpholinos (A) or morpholinos directed against nkx2.2a, nkx2.2b and nkx2.9 mRNA (B). gata3-expressing cells are not detectable in the lateral floor plate (arrow) in triple morphants, whereas dorsally located gata3-expressing cells (arrowhead) are present. (C,D) Embryos injected with a mismatch control morpholino (C) or a morpholino directed against tal2 mRNA (D). tal2 knockdown does not affect gata3 expression, indicating that tal2 does not regulate gata3. (E,F) Embryos were injected with a mismatch morpholino (E) or a morpholino directed against tal2 mRNA (F). gata2 expression is not abolished by knockdown of tal2 expression. Embryos were 24 hpf. Anterior left, dorsal up. Scale bar: 25 μm.