Fig. 7

Fig. 7 Genetic interaction between Cav-1 and β-catenin in zebrafish embryos. (A) Percentage of ventralized embryos after injection of 300 pg capped mRNA for Cav-1α or its mutants at one-cell stage. (B) Expression patterns of dorsoventral genes in embryos injected with 300 pg capped mRNA for Cav-1α or its mutants at one-cell stage. Developmental stages are shown at the bottom. Animal pole views of embryos at shield stage with the dorsal toward the right for chordin and eve1. Arrows point to the margins of expressed eve1. Dorsal views of embryos at shield stage with the animal pole toward the top for gsc and boz. Lateral views of embryos at shield stage with the dorsal toward the right for bmp2b. Dorsoposterior views of embryos at 12 hpf with the posterior toward the top for otx2. Lateral views of embryos at 24 hpf with the anterior to the left for gata1. (C) Statistical data for (B). Total numbers of embryos detected are indicated at the bottom. (D) Percentage of dorsalized embryos caused by injection of 150 pg β-cat mRNA plus 150 pg GFP mRNA, 150 pg β-cat mRNA plus 150 pg cav-1 mRNA, 150 pg β-cat-M mRNA plus 150 pg GFP mRNA, or 150 pg β-cat-M mRNA plus 150 pg cav-1 mRNA. (E) Expression patterns of dorsoventral genes in embryos from (D). (F) Percentage of normal embryos in (E) that were rescued by injection of 150 pg mRNA for cav-1α or its mutants. Total number of embryos examined was indicated at the bottom.