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Fig. 1 Transcriptional regulation of zebrafish cav-1 expression. Transcriptional expression of cav-1α and -1β was detected with whole-mount in situ hybridization (A) and real-time RT-PCR (B). Embryos at one-cell stage were injected with 300 pg frzb mRNA, 300 pg β-catenin 1 (β-cat) mRNA, 300 pg truncated bmpR mRNA, 300 pg bmp4 mRNA, 150 pg frzb mRNA plus 150 pg bmp4 mRNA, or 150 pg β-cat mRNA plus 150 pg truncated bmpR mRNA. Wild-type (WT) embryos were used as the control. Embryos at shield stage are shown in lateral views with the animal pole toward the top and the dorsal toward the right. Embryos injected with frzb mRNA and truncated bmpR mRNA were also oriented for an animal pole view with the dorsal toward the right. Real-time RT-PCR data represent mean ± S.D. from three independent experiments. *indicates p < 0.05 vs. wt and * *indicates p < 0.01 vs. wt.
Reprinted from Developmental Biology, 344(1), Mo, S., Wang, L., Li, Q., Li, J., Li, Y., Thannickal, V.J., and Cui. Z., Caveolin-1 regulates dorsoventral patterning through direct interaction with beta-catenin in zebrafish, 210-223, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.