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Fig. 2 smo- mutant embryos have no anterior neurocranium precartilage condensations. Embryos were stained with RNA probe to the precartilage marker sox9a, the pharyngeal arches were dissected away from the embryos and ventral views of the neurocranium were imaged. (A) At 48 hpf, sox9a-positive cells prefigure the morphology of the 4 dpf neurocranium in wild-type embryos. (B) Embryos lacking all Hh signaling have a complete loss of anterior neurocranium precartilage condensations; however, immediately posterior precartilage condensations are present (arrows in A,B). EP, ethmoid plate; TR, trabeculae; WT, wild type. Anterior is leftwards. Scale bar: 50 μm.