ZFIN Image: Freisinger et al., 2010, Fig. 2

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Figure Caption

Fig. 2 rgs3 inhibits wnt5b-induced Ca²⁺ dynamics.

Schematic representation illustrating that Wnt5b overexpression results in intracellular calcium release in a G protein dependent manner (A, left side) and the predicted negative effect overexpression of Rgs3 will have on the Wnt/calcium pathway (A, right side). Representative Ca²⁺ release profiles (composite image) of wnt5-overexpressing (B,D,E) and wt (C) blastula stage zebrafish embryos. (B–E) are composites of fura-2 ratiometric imaging time course showing total calcium release activity as peaks and colors mapped topographically. Ca²⁺ release profile of an embryo uniformly expressing wnt5b (B). Wt Ca²⁺ release profile (C). wnt5b expressing embryo with localized TxR/rgs3 (D) or Txr/rgs3^N109A (E). Corresponding fluorescent images illustrate the location of TxR/rgs3 (F) and TxR/rgs3^N109A (G).

Acknowledgments

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