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Fig. 8 Uprocessed ProVEGF-C inhibits VEGF-C-induced ZF4 cells proliferation.
(A) ZF4 cells were transiently transfected with empty vector (None), vector containing the wild-type (wt, HSIIRR) or mutated (mut, HSIISS) proVEGF-C cDNA. proVEGF-C processing was analyzed by western blot. Results are representative of three experiments. (B) ZF4 cells were serum deprived overnight and then treated for 24 h with media derived from LoVo cells transiently transfected with empty vectors (Control) or pSecTagB containing wild-type (wt) or mutated (mut) proVEGF-C cDNA. (C) ZF4 cells were serum deprived overnight and then treated or not for 6 h with media derived from LoVo cells transiently transfected with vector containing mut VEGF-C cDNA and incubated for 24 h with media derived from LoVo cells transfected with vector containing wt proVEGF-C cDNA. Cell proliferation was evaluated using the Cell Titer96 non-radioactive cell proliferation assay. Data are shown as means ± S.E. of three experiments performed in triplicate. *p<0.005; **p<0.0001.