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Fig. 3 Requirement of the PCs in VEGF-C-mediated Akt and ERK phosphorylation in ZF4 cells.
Confluent ZF4 cells were serum starved for 24–48 h and then treated for 2 min at 37°C with media derived from LoVo cells transiently cotransfected with empty vectors and pSecTagB vector containing proVEGF-C construct (Control) or pSecTagB vector containing proVEGF-C cDNA and pIRES2-EGFP vector expressing full-length Furin, PACE4, PC5 or PC7 cDNAs. Equal amounts of cell lysates were subjected to Western blotting using an anti-phospho-Akt (A), or an anti-phospho-ERK (B). The anti-Akt and anti-ERK were used for data normalization. Results are representative of three experiments.