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Fig. S2 Embryonic expression of tgfβ3. Whole-mount lateral (A–C) and dorsal (D–F) views are shown. At the 21-somite stage, tgfβ3 is expressed in the lens, migrating neural crest cells and notochord (A, D). Double-color in situ hybridization with known migrating neural crest marker, dlx2a, shows that tgfβ3 is expressed in a subpopulation of the migrating neural crest cells (A, D). The expression of tgfβ3 in the migrating neural crest cells intensifies in streams 2 and 3 at the 26-somrte stage (B, E) and in 24 hpf (C, F) embryos.
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Reprinted from Mechanisms of Development, 128(7-8), Cheah, F.S., Winkler, C., Jabs, E.W., and Chong, S.S., tgfbeta3 Regulation of Chondrogenesis and Osteogenesis in Zebrafish is Mediated Through Formation and Survival of a Subpopulation of the Cranial Neural Crest, 329-344, Copyright (2010) with permission from Elsevier. Full text @ Mech. Dev.