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Fig. 1 A Gli2a-GFP fusion protein is capable of rescuing adaxial F59 expression in you-too (yot) mutant embryos. Newly fertilised eggs from a yot/+ incross were injected with modified BAC DNA containing GFP tagged Gli2a. The injected embryos were fixed at the 12 somite stage and labelled with anti-F59 (red) in order to detect slow lineage muscle fibres (a), the expression of which is eliminated in yot mutant embryos (b). F59 expression was restored specifically in the Gli2a-GFP expressing cells of transient transgenic mutant embryos (c), indicating that GFP tagged Gli2a protein is functional. Dorsal view, anterior to the left. Nuclei: DAPI in blue. Scale bar: 20 μm