ZFIN Image: Devireddy et al., 2010, Fig. S2

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Figure Caption

Fig. S2 Additional Experiments, Related to Figure 2

(A) Expression of Bdh2 in mouse adult tissue and embryos. RT-PCR analysis monitoring expression of Bdh2 or, as a loading control, Gapdh.

(B) Identification of putative EntE and EntF homologs in mouse, human and zebrafish. Multiple sequence alignment of murine, human, zebrafish and bacterial homologs of EntE (left) and EntF (right). Regions of identity are indicated in gray. Only partial sequence of the 1293 amino acid bacterial EntF protein is shown.

(C) SDS-PAGE analysis of purified recombinant wild-type and mutant murine BDH2 and bacterial EntA proteins. Purified recombinant wild-type and mutant BDH2 and EntA proteins were fractionated on an SDS-polyacrylamide gel and detected by Coomassie blue staining. Molecular weight markers are shown on the right, in kDa.

Acknowledgments

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Reprinted from Cell, 141(6), Devireddy, L.R., Hart, D.O., Goetz, D.H., and Green, M.R., A mammalian siderophore synthesized by an enzyme with a bacterial homolog involved in enterobactin production, 1006-1017, Copyright (2010) with permission from Elsevier. Full text @ Cell