ZFIN Image: Devireddy et al., 2010, Fig. 2

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Figure Caption

Fig. 2 Identification of a Murine Homolog of Bacterial EntA that Mediates 2,5-DHBA Synthesis

(A) Multiple sequence alignment of EntA/BDH2 homologs. Identical residues between all five proteins are indicated in yellow, conserved residues in green, and semiconserved residues in blue.

(B) RT-PCR (left) and immunoblot (right) analysis monitoring murine Bdh2 expression in FL5.12 cells treated with one of two unrelated shRNAs targeting Bdh2 or a control nonsilencing (NS), enhanced green fluorescent protein (EGFP), or luciferase (Luc) shRNA.

(C) GC-MS analysis of the eluted 24p3 sample prepared from parental FL5.12 cells or FL5.12 cells expressing a Bdh2 shRNA.

(D) Wild-type and mutant bacterial EntA and mouse BDH2 proteins were analyzed for their ability to convert 2,3-diDHBA to 2,3-DHBA (left) or NAD to NADH (right). Error bars indicate the SD. Mutant EntA/BDH2 proteins were expressed at comparable levels to the wild-type proteins (Figure S2C).

See also Figure S2.

Acknowledgments

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Reprinted from Cell, 141(6), Devireddy, L.R., Hart, D.O., Goetz, D.H., and Green, M.R., A mammalian siderophore synthesized by an enzyme with a bacterial homolog involved in enterobactin production, 1006-1017, Copyright (2010) with permission from Elsevier. Full text @ Cell