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Fig. S5 Assessment of the Purity of Mitochondrial Preparations, Related to Figure 6
Immunoblot analysis monitoring the levels of COX4 (a mitochondrial protein), SAM68 (a nuclear protein) and actin (a cytoplasmic protein) in FL5.12 cells treated with one of two unrelated shRNAs targeting Bdh2 or a control enhanced green fluorescent protein (EGFP), luciferase (Luc) or non-silencing (NS) shRNA. The results show that the mitochondrial preparations used in the experiments presented in Figure 6 are free of cytoplasmic and nuclear contaminants.
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Reprinted from Cell, 141(6), Devireddy, L.R., Hart, D.O., Goetz, D.H., and Green, M.R., A mammalian siderophore synthesized by an enzyme with a bacterial homolog involved in enterobactin production, 1006-1017, Copyright (2010) with permission from Elsevier. Full text @ Cell