ZFIN Image: Insinna et al., 2010, Fig. 2

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Figure Caption

Fig. 2 The cnb locus encodes dync1h1. (A) Map of the cnb critical genomic region and exon/intron representation of dync1h1. Linked microsatellite markers and recombinants are placed onto the ZV7 zebrafish genomic map. (B) RT-PCR results from two independent cDNA samples each of cnb or wild-type embryos. Top image of bands from reactions with dync1h1 primers and bottom image of bands from reactions with EF1alpha primers. (C) Chromatograms showing wild-type (top) and cnb sequence flanking the C-to-A mutation. (D) Schematic of the Dync1h1 protein and location of the nonsense mutation. (E) Location of retrovirus insertion for dync1h1 mutant allele hi3684.

Acknowledgments

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