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Fig. 5 Knockdown and overexpression (OE) of sept9a_tv1 results in an increase in apoptotic cells in the tail.
Embryos at the one-cell stage were injected with 2.5 ng of MO2 or 4 pg of sept9a_tv1 mRNA and analyzed for acridine orange (AO) staining at 24 hpf. A–C: The tail fin of wild-type embryos is negative for AO indicating few apoptotic cells. D–I: Both class II MO2 and sept9a_v1 OE embryos show an increase in AO staining indicating increased cell death.
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