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Fig. 6 Involvement of GPER in zebrafish oocyte maturation. (A) Effect of E2 on GVBD of defolliculated full-grown oocytes treated with IgG and fish GPER antibody (1:300). ∗, P < 0.05 compared to Veh. (B) Effect of E2 (20 nM) on GVBD of LV oocytes (∼ 550 μm) that had been micro-injected with control (CTL) and ERα antisense morpholino oligos. AS-ER, antisense ERα oligos. ∗, +, P < 0.05 compared to vehicle (Veh). Gel image, RT-PCR detection of ERα mRNA levels in the micro-injected oocytes. M, DNA size marker. C1 and C2, control 1 and 2. A1 and A2, antisense oligos. Atn, actin control. (N = 3).
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Reprinted from Developmental Biology, 342(2), Pang, Y., and Thomas, P., Role of G protein-coupled estrogen receptor 1, GPER, in inhibition of oocyte maturation by endogenous estrogens in zebrafish, 194-206, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.