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Fig. 4 Effects of enzymatic and manual removal of ovarian follicle cells (defolliculation) on oocyte maturation. (A) Intact ovarian follicles stained with DAPI, before and after collagenase treatment. int, intact follicle; df, defolliculated follicles. (B) Effects of E2 (100 nM) on the GVBD of intact (int) and enzymatically defolliculated (df) oocytes after 6 h of incubation. (C) Effects of 100 nM E2 and G-1 on GVBD of manually defolliculated oocytes. ab, cd, ef: P < 0.05. df, manually defolliculated oocytes. (N = 3).
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Reprinted from Developmental Biology, 342(2), Pang, Y., and Thomas, P., Role of G protein-coupled estrogen receptor 1, GPER, in inhibition of oocyte maturation by endogenous estrogens in zebrafish, 194-206, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.