ZFIN Image: Insinna et al., 2010, Fig. S2

Image

Figure Caption

Fig. S2 Morpholino knock-down of dnct1b. (A, B) Ocular histology of control (A) and dnct1b (B) morphants at 3 dpf. (C) RT-PCR analysis of dnct1b morpholino-injected and control morpholino-injected embryos indicating efficient targeting of the transcript. Primers in the top image bind to regions flanking the morpholino-targeted splice junction. (D) RT-PCR analysis of dnct1b and control morphants. Primers in the top image bind to the 5′ untranslated region of dnct1b. Bottom images in (C, D) show results using control EF1alpha primers that indicate the dnct1b morpholino destabilizes the targeted mRNA. The age of embryos used to make cDNA is shown at the top of (C, D). Scale bar: 40 μm in (A, B).

Acknowledgments

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